F(ab')2 Anti-Mouse IgG F(ab')2 Pre-Adsorbed Secondary Antibody

F(ab')2 Antibody was generated by enzymatic cleavage and subsequent separation from the Fc fragment. Because of their smaller size, F(ab)2 fragments offer several advantages over intact antibodies for use in certain immunochemical techniques and experimental applications. F(ab)2 fragments penetrate into tissue samples and show better antigen recognition and signal generation in IHC. F(ab)2 fragments lack the Fc region and therefore do not bind Fc receptors which effectively lowers background staining. F(ab')2 Antibody is ideal for investigators who routinely perform flow cytometry, immunohistochemistry or IHC and other immunoassays.

Goat Polyclonal, Unconjugated

1.0 mg/mL by UV absorbance at 280 nm

ELISA 1:20, 000-1:100, 000; Western Blot 1:500-1:5, 000; Immunochemistry 1:1, 000-1:5, 000

Mouse

IgG F(ab')2

None

Wet Ice

Store vial at 4° C prior to opening.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.  For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.