Anti-mGluR1 (extracellular)-ATTO Fluor-488

Antibodies

ATTO-488. Maximum absorption 501 nm; maximum fluorescence 523 nm. The fluorescence is excited most efficiently in the 480 - 515 nm range. This label is analogous to the dye fluorescein isothiocyanate (FITC) and can be used with filters used to detect FITC.

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

Lyophilized powder

Metabotropic glutamate receptor 1, GRM1, mGlu1, GPRC1a, PPP1R85, SCAR13 - A Rabbit Polyclonal Antibody to Metabotropic Glutamate Receptor 1 Conjugated to the Fluorescent Dye ATTO-488

Mouse, human, dog - identical

Confirmed by amino acid analysis and mass spectrometry

1 mg/ml

Extracellular, N-terminus

Rat

41116161

L-Glutamate is the major excitatory neurotransmitter in the central nervous system. It operates through several receptors that are categorized as ionotropic (ligand-gated cation channels) or metabotropic (G-protein coupled receptors). The metabotropic glutamate receptor family includes eight members (mGluR1-8) that have been divided into three groups based on their sequence homology, pharmacology and signal transduction.Group I of the metabotropic glutamate receptors includes the mGluR1 and mGluR5 receptors. The receptors present the typical G-protein coupled receptor (GPCR) signature topology: seven transmembrane domains with a large extracellular N-terminus and an intracellular C-terminus. The N-terminus domain of Group I receptors contains the glutamate binding site while the cytoplasmic C-terminus domain has an important role in the regulation of receptor activity through interactions with other proteins such as the Homer adaptor proteins.mGluR1 and mGluR5 receptors signal through Gq/G11, that activates phospholipase C and ultimately produces an increase in inositol trisphosphate and cytosolic Ca2+. Additional downstream signaling pathways include the activation of PKC and modulation of Ca2+ and K+ ion channels. Activation of signaling pathways independent of G-proteins have also been reported.mGluR1 is predominantly expressed in nervous tissue although expression in several non-neural cell types has also been described. In the brain it is highly expressed in the hippocampus, cerebellum, olfactory bulb and thalamus.The mGluR1 receptor is involved in several physiological processes such as neuronal development, induction of long-term potentiation (LTP) and depression (LTD) as well as in pathological disorders such as brain trauma, chronic pain, Parkinson's and Huntington's disease.