Anti-PTH2R-ATTO Fluor-488

Antibodies

ATTO-488. Maximum absorption 501 nm; maximum fluorescence 523 nm. The fluorescence is excited most efficiently in the 480 - 515 nm range. This label is analogous to the dye fluorescein isothiocyanate (FITC) and can be used with filters used to detect FITC.

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

Lyophilized powder

Parathyroid hormone 2 receptor, PTH2 receptor, PTHR2 - A Rabbit Polyclonal Antibody to Parathyroid Hormone 2 Receptor Conjugated to the Fluorescent Dye ATTO-488

Mouse - identical; human - 11/13 amino acid residues identical

Confirmed by amino acid analysis and mass spectrometry

1 mg/ml

Extracellular, N-terminus

Rat

41116161

PTH (parathyroid hormone) regulates Ca2+ and phosphate homeostasis through its action on specific receptors in kidney and bone. There are two known PTH receptors in humans, PTH receptor type 1 (PTH1 receptor) and PTH receptor type 2 (PTH2 receptor)1.The PTH2 receptor is a member of a class B subfamily of G-protein-coupled receptors that includes the receptors for the glucagon-GHRH-VIP family of peptides (glucagon, GLP-I, GIP, GHRH, VIP, secretin, PACAP) and for calcitonin and CRF2. PTH2 receptor shares the same basic structure of the GPCR superfamily of proteins. A defining feature of the family B receptors is the relatively long extracellular N-terminal domain, which comprises about 150 amino acids and is important for ligand binding. The seven transmembrane domains are believed to be arranged in a circular or oval configuration, as seen in rhodopsin. The transmembrane domains are connected by three extracellular and three intracellular loops, and a C-terminal tail of about 130 amino acids extends intracellularly3.Northern blots of human messenger RNA (mRNA) show that the PTH2 receptor is most highly expressed in the central nervous system, preferentially in the hypothalamic regions and is also detected in the pancreas, testis, placenta, and lung4. Recent data support that PTH2 receptor is involved in hypothalamic releasing-factor secretion and pain5.