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Hepatitis C Virus (HCV) Core Protein

ArtNr ANOG-MO-I40015A
Hersteller Anogen
Menge 0.5mg
Kategorie
Typ Antibody Monoclonal
Format Aff. Pur
Applikationen ELISA
Clon A1/3D1
Specific against Human (Homo sapiens)
Host Mouse
Isotype IgG1 kappa
Protein Familie Infectious Agents
ECLASS 10.1 42030590
ECLASS 11.0 42030590
UNSPSC 12352203
Similar products HCV
Lieferbar
Description
Mouse monoclonal antibody to human hepatitis C virus (HCV) core protein
Target Protein
Human hepatitis C virus (HCV) core protein
Immunogen
Synthetic peptides derived from HCV core protein
Specificity
mAb A1/3D1 is reactive to recombinant core protein C + envelope protein M (residues 1-142 on HCV polyprotein) and synthetic core protein C (residues 1-61 on HCV polyprotein).
Purification
Protein G affinity purified
Species Reactivity
Human hepatitis C virus, others not tested
Cross Reactivity
It showed no cross reaction with recombinant or synthetic HCV non-structural proteins (NS-3 and NS-4).
Myeloma
Sp2/0-Ag14
Background
Hepatitis C virus (HCV) causes chronic hepatitis and liver cirrhosis in human through blood and body fluid transmission. HCV has a positive sense single RNA genome enclosed in the nucleocapsid made of Core Protein (Capsid Protein). The nucleocapsid is covered by an envelope made of lipoproteins (E1 and E2). The 9.6 kb HCV genome has a single open-reading frame, which is to be translated into a single polyprotein. HCV viral proteins are produced after processing the polyprotein. Genes for core protein and envelop proteins are located adjacently at the 5’-end of HCV genome, followed by genes for non-structural proteins including NS2, NS3, NS4A, NS4B, NS5, NS5A and NS5B.
Formulation
Lyophilized from a solution in 0.01M PBS, pH 7.2
Reconstitution
Double distillated water is recommended to adjust the final concentration to 1.00mg/mL.
Application info
ELISA: mAb A1/3D1 was tested using indirect ELISA method. The testing plates were coated with HCV non-structural protein (NS), core protein (C) and mixture of NS and C respectively. The neat culture supernatant of hybridoma showed a strong reactivity with HCV capsid protein (OD492 value > 2.0) and with mixture of NS and C (OD492 value >1.5), and showed no reactivity with HCV non-structural protein (OD492 value < 0.03). mAb A1/3D1 recognizes in-vitro translated HCV core protein.
Not suitable for use in Western Blot
Research Area
Virology
Storage
Store at -20C

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Menge: 0.5mg
Lieferbar: In stock
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