Anti-CD169 Antibody [3D6.112]

Store undiluted at -20°C only. Storage in frost free freezers is not recommended. Avoid freeze-thaw cycles. Should this product contain a precipitate we recommend microcentrifugation before use.

Monoclonal

1 mg/ml

Protein G affinity chromatography of tissue culture supernatant.

This antibody recognises mouse CD169 also known as sialoadhesin, Sheep erythrocyte receptor or Siglec-1. CD169 is a 1695 amino acid, ~180 kDa single pass, type 1 transmembrane glycoprotein containing a single Ig-like V-type domain and sixteen Ig-like C2-type domains. CD169 is a macrophage restricted receptor, preferentially binding to alpha 2, 3 linked sialic acid residues and is expressed on stromal macrophages in many tissues, particularly in lymph nodes, bone marrow and on marginal metallophilic macrophages in the spleen. CD169 has been implicated in a number of roles including cell-cell interactions with lymphocytes and granulocytes. CD169 expressing macrophages have also been suggested to play a role in host resistance to lymphoma metastasis. In pigs CD169 has also been identified as a macrophage restricted receptor for porcine reproductive and respiratory syndrome virus. CD169 expressing macrophages have also been implicated in the regulation of autoimmune disease progression through their interaction with regulatory T cells via CD169. CD169 has also been shown to play a critical role in the recognition and elimination of invasive sialylated microorganisms including Campylobacter jejuni and group B Streptococcus. The functional activity of rat anti mouse CD169 antibody, clone 3D6.112, its ability to inhibit binding of red blood cells to CD169 can be considerably enhanced by derivitization of the antibody with polyethylene glycol.