Anti-Feline Immunodeficiency Virus gp95 Antibody [SU1-30]

Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.

Monoclonal

Supplied in Phosphate Buffered Saline with <0.1% Sodium Azide.

Use 10µl of the suggested working dilution to label 10⁶ cells in 100µl

This antibody recognises the relatively conserved E60 peptide (amino acid sequence SSWKQRNRWEWRPDF) located within the V3 region of FIV (Feline Immunodeficiency Virus) envelope glycoprotein SU, gp95. The primary receptor for FIV infection of cats is the T-cell activation antigen CD134 which, along with CXC chemokine receptor 4 (CXCR4), facilitates the infection of CD4+ primary target cells, resulting in a depletion of the CD4+ population. The V3 loop of SU, gp95 is a major immunogenic domain, suggested to contain the main determinants for the binding of FIV SU, gp95 to CXCR4. Mouse anti Feline Immunodeficiency Virus gp95 antibody, clone SU1-30 inhibits the sCD134-activated entry of both clade B and clade C FIV strains (de Parseval, A. et al. 2006). Mouse anti Feline Immunodeficiency Virus gp95 antibody, clone SU1-30 does not recognize Feline Leukaemia Virus, Feline Herpes Virus type 1, Feline Coronavirus or Feline Calicivirus. Removal of sodium azide is recommended prior to use in functional assays.