Anti-CD197 Antibody

Store undiluted at -20°C only. Storage in frost free freezers is not recommended. Avoid freeze-thaw cycles. Should this product contain a precipitate we recommend microcentrifugation before use.

Polyclonal

1 mg/ml

ELISA: 1:100, 000, Flow Cytometry: 1:10, Functional Assays: 8ug, IHC-P: 1:250, WB: 1:1, 000, Use 10µl of the suggested working dilution to label 1x10⁶ cells in 100µl.

This antibody recognises mouse C-C chemokine receptor type 7, also known as CD197, CCR7, Epstein-Barr virus-induced G-protein coupled receptor 1 or MIP-3 beta receptor. CD197 is a 378 amino acid ~43 kDa multi pass type III transmembrane glycoprotein and member of the G-protein coupled receptor 1 family (UniProt: P74447). CD197 is expressed by various lymphoid tissues, activated B and T cells, naive T cells, NK cells and dendritic cells (DCs), which acts a high-affinity receptor for the chemokines MIP-3beta (CCL19) and CCL21.Ligand binding of CD197 plays a vital role in the homing of naive T cells and antigen-loaded DCs into secondary organs. Expression of CD197 is strongly up-regulated in B cells infected with Epstein-Barr virus (EBV) and T cells infected with herpesvirus 6 or 7. Goat anti Mouse CD197 antibody can be used to distinguish between central memory and effector memory T cells and is suitable for use in immunocytochemistry.