Cytochrome C (Mitochondrial Marker)(6H2.B4), 1mg/mL

Primary

Primary Antibodies Only

Purified, BSA-free

Room temperature

15 kDa

Cytochrome c is a well-characterized mobile electron transport protein that is essential to energy conversion in all aerobic organisms. In mammalian cells, this highly conserved protein is normally localized to the mitochondrial inter-membrane space. More recent studies have identified cytosolic cytochrome c as a factor necessary for activation of apoptosis. During apoptosis, cytochrome c is trans-located from the mitochondrial membrane to the cytosol, where it is required for activation of caspase-3 (CPP32). Overexpression of Bcl-2 has been shown to prevent the translocation of cytochrome c, thereby blocking the apoptotic process. Overexpression of Bax has been shown to induce the release of cytochrome c and to induce cell death. The release of cytochrome c from the mitochondria is thought to trigger an apoptotic cascade, whereby Apaf-1 binds to Apaf-3 (caspase-9) in a cytochrome c-dependent manner, leading to caspase-9 cleavage of caspase-3.

Mus musculus (mouse)

Stable at room temperature or 37°C (98°F) for 7 days.

Primary and secondary antibodies for multiple methodology
immunostaining detection application

1 mg/mL

Guaranteed for at least 24 months from date of receipt when stored as recommended

Monoclonal

CYCS

K-562, HL-60, Jurkat, NIH3T3 or PC-3 cells. Liver or Cardiac muscle.

Flow, intracellular (verified), IF (verified)

Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody|Flow cytometry: 0.5-1 ug/million cells|Immunofluorescence: 0.5-1 ug/mL|Optimal dilution for a specific application should be determined by user

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