Ep-CAM / CD326(HEA125), 1mg/mL

Primary

Primary Antibodies Only

Purified, BSA-free

Room Temperature (Next day)

40-43 kDa

Note: All CF® Dye and biotin conjugates of this antibody have been discontinued. The antibody is still available unlabeled, with or without BSA.This antibody recognizes a 40-43 kDa transmembrane epithelial glycoprotein, identified as epithelial specific antigen (ESA), or epithelial cellular adhesion molecule (Ep-CAM). It is expressed on baso-lateral cell surface in most simple epithelia and a vast majority of carcinomas with the exception of adult squamous epithelium, hepatocytes and gastric epithelial cells. Antibody to Ep-CAM has been used to distinguish adenocarcinoma from pleural mesothelioma and hepatocellular carcinoma. It is also useful in distinguishing serous carcinomas of the ovary from mesothelioma. It has been reported that this epithelial antigen plays an important role as a tumor-cell marker in lymph nodes from patients with esophageal carcinoma otherwise classified as node-negative.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Mus musculus (mouse)

Stable at room temperature or 37°C (98°F) for 7 days.

Primary and secondary antibodies for multiple methodologyimmunostaining detection application

1 mg/mL

Guaranteed for at least 24 months from date of receipt when stored as recommended

Monoclonal

TACSTD1

HT29 cells or Ovarian carcinoma

Epithelial cells

IHC, frozen (published)|Flow, surface (verified)|IF (verified)|IHC, FFPE (verified)|IP (published)|RIA (published)

Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody|Immunohistology (formalin): 0.5-1 ug/mL|Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min

Adenocarcinoma-associated Antigen , Cell Surface Glycoprotein Trop-1 , Chromosome 17 , ECS-1 , EGP2 , EGP314 , EGP40 , Epidermal Surface Antigen 1 , Epithelial Cell Adhesion Molecule , Epithelial Glycoprotein 314 , ESA , ESA1 , FLOT2 , Flotillin-2 , KSA , Membrane Component , REG-1 , Reggie-1 , Reggie-2 , Surface Marker-1 (M17S1) , TACD1 , TROP1 , Tumor-associated Calcium Signal Transducer 1 (TACSTD1)

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