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NSE gamma (Neuron Specific Enolase, gamma) (Neuroendocrine Marker) (ENO2/1462), 1mg/mL

ArtNr B-BNUM1462-50
Hersteller Biotium
Menge 50 uL
Kategorie
Typ Antibody Monoclonal
Format Liquid
Applikationen IHC
Clon ENO2/1462
Specific against Human (Homo sapiens)
Host Mouse
Isotype IgG2b
ECLASS 10.1 42030590
ECLASS 11.0 42030590
UNSPSC 12352203
Alias 2-phospho-D-glycerate hydrolyase,ENO2,ENOG,Enolase 2 gamma neuronal,Enolase2,Gamma-enolase,Neural enolase,Neuron specific gamma enolase,Neuron-specific enolase,NSE
Versandbedingung Raumtemperatur
Lieferbar
Manufacturer - Type
Primary
Manufacturer - Applications
IHC, FFPE (verified)
Manufacturer - Category
Primary Antibodies Only
Manufacturer - Targets
NSE-Gamma
Manufacturer - Conjugate / Tag
Purified, BSA-free
Manufacturer - Host
Mus musculus (mouse)
Shipping Temperature
Room Temperature (Next day)
Storage Conditions
Store at -10 to -35 °C|Protect fluorescent conjugates from light
Molecular Weight
~50 kDa
Manufacturer - Research Area
Cancer|Metabolism|Neuroscience
Description
This antibody recognizes a protein of about 50 kDa, which is identified as gamma-enolase. Three isoenzymes of enolases are identified, alpha, beta and gamma. Alpha-isoform is expressed in most tissues, whereas beta-form is expressed predominantly in muscle tissue whereas gamma-enolase is found only in nervous tissue. These isoforms exist as both homodimers and heterodimers, and they play a role in converting phosphoglyceric acid to phosphenolpyruvic acid in the glycolytic pathway. NSE-gamma is a useful marker to identify peripheral nerves and tumors of neuro-endocrine origins, such as pheochromocytomas. It it be usually employed in combination with other markers such as Synaptophysin, Chromogranin A, and Neurofilament.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
Product origin
Animal
Ingredient of biological origin
Mus musculus (mouse)
Undated stability guarantee in PI
2 years
Stability at RT during shipping (protected from light)
Stable at room temperature or 37°C (98°F) for 7 days.
UNSPSC Commodity
41116161
UNSPSC Commodity Title
Primary and secondary antibodies for multiple methodologyimmunostaining detection application
Classified or regulated chemicals
PBS, no BSA, no sodium azide (CAS 26628-22-8)
Concentration
1 mg/mL
Storage buffer
PBS, no BSA, no azide
Dated shelf life printed on label
Guaranteed for at least 24 months from date of receipt when stored as recommended
Immunogen (Secondaries, for anti tag only)
A synthetic peptide corresponding to aa416-433 of human NSE gamma (exact sequence is proprietary)
Clonality
Monoclonal
Regulatory Status
For research use only (RUO)
Human Gene Symbol
ENO2
Unigene
511915
Positive Control
HepG2, SH-SY-5Y, HeLa or Y79 cells. Pancreas, Cerebellum or Pheochromocytoma.
Antibody target cellular localization
Cytoplasmic
Cell tissue expression
Neurons
Tumor expression
Neuroendocrine cancer
Validated in protein array
Monospecific
Antibody applications
IHC, FFPE (verified)
Verified antibody applications
IHC (FFPE) (verified)
Antibody application notes
Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody|Immunofluorescence: 1-2 ug/mL|Immunohistology (formalin) 0.1-0.2 ug/mL|Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min|Flow Cytometry 0.5-1 ug/million cells/0.1 mL|Optimal dilution for a specific application should be determined by user
Manufacturer - Antibody Reactivity
NSE-Gamma
Antibody number
#1462

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

Menge: 50 uL
Lieferbar: In stock
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