« Zurück
Startseite /
Anti-DDX5 Antibody PicobandÂ® (monoclonal, 3F9)

Anti-DDX5 Antibody PicobandÂ® (monoclonal, 3F9)

ArtNr	BOS-M00670-1
Hersteller	Boster
Menge	100 ug/vial
Kategorie	Antibodies Primary Antibodies
Typ	Antibody Monoclonal
Format	Lyophilized
Applikationen	WB, FC, IF, IHC, ICC, IHC-F
Clon	3F9
Specific against	Human (Homo sapiens), Mouse (Murine, Mus musculus), Rat (Rattus norvegicus)
Host	Mouse
Isotype	IgG2b
Sensitivity	>5000 cells
Citations	1. Brody, L. C., Abel, K. J., Castilla, L. H., Couch, F. J., McKinley, D. R., Yin, G.-Y., Ho, P. P., Merajver, S., Chandrasekharappa, S. C., Xu, J., Cole, J. L., Struewing, J. P., Valdes, J. M., Collins, F. S., Weber, B. L. Construction of a transcription map surrounding the BRCA1 locus of human chromosome 17. Genomics 25: 238-247, 1995. 2. Caretti, G., Schiltz, R. L., Dilworth, F. J., Di Padova, M., Zhao, P., Ogryzko, V., Fuller-Pace, F. V., Hoffman, E. P., Tapscott, S. J., Sartorelli, V. The RNA helicases p68/p72 and the noncoding RNA SRA are coregulators of MyoD and skeletal muscle differentiation. Dev. Cell 11: 547-560, 2006. 3. Iggo, R., Gough, A., Xu, W., Lane, D. P., Spurr, N. K. Chromosome mapping of the human gene encoding the 68-kDa nuclear antigen (p68) by using the polymerase chain reaction. Proc. Nat. Acad. Sci. 86: 6211-6214, 1989.
ECLASS 10.1	42030590
ECLASS 11.0	42030590
UNSPSC	12352203
Alias	Probable ATP-dependent RNA helicase DDX5; DEAD box protein 5; RNA helicase p68; DDX5; G17P1, HELR, HLR1
Lieferbar
Manufacturer - Category	Primary Antibodies
Manufacturer - Isotype	Mouse IgG2b
Storage Conditions	Store at -20Â°C for one year from date of receipt. After reconstitution, at 4Â°C for one month. It can also be aliquotted and stored frozen at -20Â°C for six months. Avoid repeated freeze-thaw cycles.
Observed Molecular Weight	71 kDa
Clonality	Monoclonal
Application Details	Western blot, 0.1-0.5?g/ml, Human, Mouse, Rat Immunohistochemistry (Paraffin-embedded Section), 0.5-1?g/ml, Human, Mouse, Rat Immunohistochemistry (Frozen Section), 0.5-1?g/ml, Human Immunocytochemistry/Immunofluorescence, 2?g/ml, Human Immunofluorescence, 2?g/ml, Human, Mouse Flow Cytometry (Fixed), 1-3?g/1x10⁶ cells, Human
Manufacturer - Gene Name	DDX5
Gene Full Name	DEAD (Asp-Glu-Ala-Asp) box helicase 5
Manufacturer - Specificity	No cross reactivity with other proteins.
Background	DDX5 (DEAD/H BOX 5), also known as HLR1 or G17P1, is an enzyme that in humans is encoded by the DDX5 gene. The p68 protein is a proliferation-associated nuclear antigen first identified through its highly specific cross-reaction with the simian virus 40 tumor antigen (Iggo et al., 1989). Subsequently, homology to eukaryotic translation initiation factor was found, and amino acid sequence blocks characteristic of a large superfamily of proteins with putative helicase activity were demonstrated. Brody et al. (1995) confirmed that this gene is located on chromosome 17 in the region of the BRCA1 gene at 17q21. By immunoprecipitation analysis, Caretti et al. (2006) found that p68, p72 (DDX17), and the noncoding RNA SRA (SRA1) associated with MYOD (MYOD1) in MYOD-transfected HeLa cells.
Immunogen	E.coli-derived human DDX5 recombinant protein (Position: R85-K328).
Contents	Each vial contains 4 mg Trehalose, 0.9mg NaCl, 0.2mg Na₂HPO₄, 0.05 mg NaN₃.
Purification	Immunogen affinity purified.
Reconstitution	Add 0.2 ml of distilled water will yield a concentration of 500 ug/ml.
Concentration	Adding 0.2 ml of distilled water will yield a concentration of 500 ?g/ml.
Manufacturer - Research Category	Cardiogenesis, Cardiovascular, 2339, DNA/RNA, Epigenetics and Nuclear Signaling, Transcription Factors/Regulators
Protein Function	Involved in the alternative regulation of pre-mRNA splicing; its RNA helicase activity is necessary for increasing tau exon 10 inclusion and occurs in a RBM4-dependent manner. Binds to the tau pre-mRNA in the stem-loop region downstream of exon 10. The rate of ATP hydrolysis is highly stimulated by single-stranded RNA. Involved in transcriptional regulation; the function is independent of the RNA helicase activity. Transcriptional coactivator for androgen receptor AR but probably not ESR1. Synergizes with DDX17 and SRA1 RNA to activate MYOD1 transcriptional activity and involved in skeletal muscle differentiation. Transcriptional coactivator for p53/TP53 and involved in p53/TP53 transcriptional response to DNA damage and p53/TP53-dependent apoptosis. Transcriptional coactivator for RUNX2 and involved in regulation of osteoblast differentiation. Acts as transcriptional repressor in a promoter-specific manner; the function probably involves association with histone deacetylases, such as HDAC1. As component of a large PER complex is involved in the inhibition of 3' transcriptional termination of circadian target genes such as PER1 and NR1D1 and the control of the circadian rhythms.
Subcellular Localization	Nucleus. Spliceosome.
Description	Boster Bio Anti-DDX5 Antibody Picoband® (monoclonal, 3F9) catalog # M00670-1. Tested in Flow Cytometry, IF, IHC, IHC-F, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Hinweis: Die dargestellten Informationen und Dokumente (Bedienungsanleitung, Produktdatenblatt, Sicherheitsdatenblatt und Analysezertifikat) entsprechen unserem letzten Update und sollten lediglich der Orientierung dienen. Wir übernehmen keine Garantie für die Aktualität. Für spezifische Anforderungen bitten wir Sie, uns eine Anfrage zu stellen.

Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

Arbeitsanleitung (Data Sheet) anfordern

BOS-M00670-1-datasheet.pdf

BOS-M00670-1-safety-datasheet.pdf