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Anti-Mouse PD-1 In Vivo Antibody - Ultra Low Endotoxin (29F.1A12) Europäischer Partner

Hersteller IchorBio
Kategorie
Typ Antibody
Specific against Mouse
Clon 29F.1A12
Isotype IgG2a Kappa
Applikationen WB, FC, IHC, Blocking
Menge 25mg
Host Rat
ArtNr ICH1091UL-25MG
Targets CD279
eClass 6.1 32160702
eClass 9.0 32160702
Lieferbar
Manufacturers Product Category
Ultra-Low Endotoxin, anti-mouse
Target Information
PD-1
Isotype Information
Rat IgG2a Kappa
Purification method
This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.
Antigen Distribution
Induced on splenic T and B lymphocytes, thymocytes, and myeloid cells after stimulation.Subset of double negative thymocytes, activated T and B cells
Background
Programmed death-1 (PD-1), also know as CD279 is a 50-55 kD glycoprotein belonging to the CD28 family of the Ig superfamily. PD-1 is transiently expressed on CD4 and CD8 thymocytes as well as activated T and B lymphocytes and myeloid cells. Like the clones RMP1-14 and J43 antibodies, the 29F.1A12 antibody has been shown to block the binding of PD-1 to its ligands in vivo.
Immunogen
PD-1 cDNA followed by PD-1-Ig fusion protein
Concentration
≥ 2.0 mg/ml
Formulation
0.01 M phosphate buffered saline (PBS) pH 7.2 - 7.4, 150 mM NaCl with no carrier protein, potassium or preservatives added.
Endotoxin
≤ 0.5 EU/mg as determined by the LAL method
Aggregation
Aggregation level ≤ 1%
IMPACT Pathogen Test
We use the IMPACT test generated by IDEXX Laboratories to guarantee our Ultra Low Endotoxin antibodies are pathogen free. Our rat antibodies are tested for: - Mycoplasma spp - Mycoplasma pulmonis - Pneumonia virus of mice - Kilham's rat virus - Toolan's H1 virus - Rat parvovirus - Lymphocytic choriomeningitis virus - Rat cytomegalovirus - Sendai virus - Rat coronavirus - Sialodacryoadenitis virus - Rat minute virus - Seoul virus - Mouse adenovirus - Reovirus 3 - Rat theilovirus
Storage
anti-PD-1 In Vivo Antibody - Ultra Low Endotoxin (29F.1A12) is stable when stored at 2-8°C for at least four (4) weeks. For long-term storage aseptically aliquot in working volumes without diluting and store at –80°C. Avoid Repeated Freeze Thaw Cycles.
Application Notes
Western Blotting: To detect Mouse PD-1 this antibody can be used at a concentration of 30 µg/ml. - Blocking: The 29F.1A12 antibody has been shown to block the binding of PD-1 to its ligands in vivo. For immune checkpoint antibodies 200 ug per mouse per injection is the standard dosage in the literature. NOTE: The response is model dependent. We have seen some tumor lines in mice that grow progressively. If we treat the animal with anti-PD-1 (days 3, 6, 9 @ 200 ug per animal per injection) then the tumor will regress, and disappear. - Each investigator should determine their own optimal working dilution for specific applications.
Use
Products are for research use only. Not for use in diagnostic or therapeutic procedures.

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

Menge: 25mg
Lieferbar: In stock
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Lieferung vsl. bis 02.05.2024 

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