Collagen Type IV IHC Antibody

Description Collagen IV is comprised of a third chain(alpha-3) together with the2 classical ones, alpha-1 and alpha-2. COL4A3 gene is localized to 2q36-q37 by analysis of somatic cell hybrids and by in situ hybridization. The alpha-3 chain of type IV collagen induces autoimmune Goodpasture syndrome. Mutations in the COL4A4 andCOL4A3 genes cause familial benign hematuria. Catalog Number IW-MA1030 Quantity 9 ml Host Mouse Clone Col-30 Isotype Mouse IgG1 Immunogen Human collagen type IV Purity Purified by the goat anti-mouse IgG affinity chromatography. Conjugate Unconjugated Species Reactivity Human, rat. Not tested in other species. Positive Control Human mammary cancer, kidney, skin. Cellular Localization Cytoplasmic Form Ready to use solution in PBS with stabilizer and 0.01% sodium azide. No further dilution needed. Serum blocking step should be omitted. Storage Store at 2-8 CC. Do not freeze. Applications IHC-P: Formalin fixed paraffin embedded tissue sections. IHC-Fr: Formalin or acetone fixed frozen sections. ICC: Not tested. Limitations This product is intended for Research Use Only. Interpretation of the test results is solely the responsibility of the user. Precautions Users should follow general laboratory precautions when handling this product. Wear personal protective equipment to avoid contact with skin and eyes. References 1. Kalluri, R.; Gattone, V. H., II; Noelken, M. E.; Hudson, B. G. : The alpha-3 chain of type IV collagen induces autoimmune Goodpasture syndrome. Proc. Nat. Acad. Sci. 91: 6201-6205, 1994. 2. Badenas, C.; Praga, M.; Tazon, B.; Heidet, L.; Arrondel, C.; Armengol, A.; Andres, A.; Morales. E.; Camacho, J. A.; Lens, X.; Davila, S.; Mila, M.; Antignac, C.; Darnell, A.; Torra, R. : Mutations in the COL4A4 and COL4A3 genes cause familial benign hematuria. J. Am. Soc. Nephrol. 13: 1248-1254, 2002.