beta-Actin IHC Antibody

Description The primary site of action of cytochalasin B on cell motility processes is beta-actin. Habets et al. (1992) generated hybrids that harbor only specific regions of human chromosome 7 and assigned the ACTB locus to 7p15-p12. ACTB and the other assigned beta-actin-related sequences are dispersed over at least four different chromosomes including one locus assigned to the X chromosome.A mutation of beta-actin that alters depolymerization dynamics is associated with autosomal dominant developmental malformations, deafness, and dystonia. Catalog Number IW-MA1115 Quantity 9 ml Host Mouse Clone AC-15 Isotype Mouse IgG1 Immunogen slightly modified beta-cytoplasmic actin N-terminal peptide, Ac-Asp-Asp-Asp-Ile-Ala-Ala-Leu-Val-Ile-Asp-Asn-Gly-Ser-Gly-Lys, conjugated to KLH. Purity Purified by the goat anti-mouse IgG affinity chromatography. Conjugate Unconjugated Species Reactivity Human, mouse, rat. Not tested in other species. Positive Control Skeletal muscle Cellular Localization Cytoplasmic Form Ready to use solution in PBS with stabilizer and 0.01% sodium azide. No further dilution needed. Serum blocking step should be omitted. Storage Store at 2-8 CC. Do not freeze. Applications IHC-P: Formalin fixed paraffin sections. IHC-Fr: Not tested. ICC: Not tested. Limitations This product is intended for Research Use Only. Interpretation of the test results is solely the responsibility of the user. Precautions Users should follow general laboratory precautions when handling this product. Wear personal protective equipment to avoid contact with skin and eyes. References 1. Ng, S.-Y.; Gunning, P.; Eddy, R.; Ponte, P.; Leavitt, J.; Kedes, L.; Shows, T. : Chromosome 7 assignment of the human beta-actin functional gene (ACTB) and the chromosomal dispersion of pseudogenes. (Abstract) Cytogenet. Cell Genet. 40: 712 only, 1985. 2. Procaccio, V.; Salazar, G.; Ono, S.; Styers, M. L.; Gearing, M.; Davila, A.; Jimenez, R.; Juncos, J.; Gutekunst, C.-A.; Meroni, G.; Fontanella, B.; Sontag, E.; Sontag, J. M.; Faundez, V.; Wainer, B. H. : A mutation of beta-actin that alters depolymerization dynamics is associated with autosomal dominant developmental malformations, deafness, and dystonia. Am. J. Hum. Genet. 78: 947-960, 2006. 3. Ng, S.-Y.; Gunning, P.; Eddy, R.; Ponte, P.; Leavitt, J.; Shows, T.; Kedes, L. : Evolution of the functional human beta-actin gene and its multi-pseudogene family: conservation of the noncoding regions and chromosomal dispersion of pseudogenes. Molec. Cell. Biol. 5: 2720-2732, 1985.