MAPK 1/3 IHC Antibody

Description MAPK1(ERK2) shares high homology with MAPK3(ERK1). MAP kinase phosphatase as a locus of flexibility in a mitogen-activated protein kinase signaling network. Mitogen-activated protein (MAP) kinases [also known as Erks] have been established to function as important mediators of signal transduction by growth factor receptors. ERK1/ERK2-dependent activation of endogenous ribosomal transcription, while inactivation of ERK1/ERK2 causes an equally immediate reversion to the basal transcription level. ERK1/ERK2 was found to phosphorylate the architectural transcription factor UBF at amino acids 117 and 201 within HMG boxes 1 and 2, preventing their interaction with DNA. Mutation of these sites inhibited transcription activation and abrogated the transcriptional response to ERK1/ERK2. Catalog Number IW-PA1049 Quantity 9 ml Host Rabbit Clone Polyclonal Isotype IgG Immunogen A synthetic peptide mapping at the N-terminal of the human MAPK1+3, identical to the related rat sequence. Purity Immunogen affinity purified Conjugate Unconjugated Species Reactivity Human, mouse, rat.Not tested in other species. Positive Control Human intestinal cancer Cellular Localization Cytoplasmic/nuclear Form Ready to use solution in PBS with stabilizer and 0.01% sodium azide. No further dilution needed. Serum blocking step should be omitted. Storage Store at 2-8 CC. Do not freeze. Applications IHC-P: Heat induced epitope retrieval is required on formalin fixed paraffin sections. IHC-Fr: Not tested. ICC: Not tested. Limitations This product is intended for Research Use Only. Interpretation of the test results is solely the responsibility of the user. Precautions Users should follow general laboratory precautions when handling this product. Wear personal protective equipment to avoid contact with skin and eyes. References 1. Bhalla, U. S.; Ram, P. T.; Iyengar, R. : MAP kinase phosphatase as a locus of flexibility in a mitogen-activated protein kinase signaling network. Science 297: 1018-1023, 2002. 2. Li, L.; Wysk, M.; Gonzalez, F. A.; Davis, R. J. : Genomic loci of human mitogen-activated protein kinases. Oncogene 9: 647-649, 1994.