PKC alpha IHC Antibody

Description Protein kinase C (PKC) is the major phorbol ester receptor. Activation of PKC by calcium ions and the second messenger diacylglycerol is thought to play a central role in the induction of cellular responses to a variety of ligand-receptor systems and in the regulation of cellular responsiveness to external stimuli. Three of these, termed alpha, beta and gamma, are highly homologous. PRKCA1 is mapped to 17q22-q23.2. PKC-alpha regulates cardiac contractility and propensity toward heart failure Catalog Number IW-PA1065 Quantity 9 ml Host Rabbit Clone Polyclonal Isotype IgG Immunogen A synthetic peptide corresponding to the C-terminal of human PKCalpha, identical to the related rat and mouse sequence. Purity Immunogen affinity purified Conjugate Unconjugated Species Reactivity Human, mouse, rat.Not tested in other species. Positive Control Human mammary cancer Cellular Localization Cytoplasmic Form Ready to use solution in PBS with stabilizer and 0.01% sodium azide. No further dilution needed. Serum blocking step should be omitted. Storage Store at 2-8 CC. Do not freeze. Applications IHC-P: Heat induced epitope retrieval enhance stain on formalin fixed paraffin sections. IHC-Fr: Not tested. ICC: Not tested. Limitations This product is intended for Research Use Only. Interpretation of the test results is solely the responsibility of the user. Precautions Users should follow general laboratory precautions when handling this product. Wear personal protective equipment to avoid contact with skin and eyes. References 1. Braz, J. C.; Gregory, K.; Pathak, A.; Zhao, W.; Sahin, B.; Klevitsky, R.; Kimball, T. F.; Lorenz, J. N.; Nairn, A. C.; Liggett, S. B.; Bodi, I.; Wang, S.; and 9 others : PKC-alpha regulates cardiac contractility and propensity toward heart failure. Nature Med. 10: 248-254, 2004. 2. Latos-Bielenska, A.; Klett, C.; Just, W.; Hameister, H. : Refinement of localization of the human genes for myeloperoxidase (MPO), protein kinase C, alpha polypeptide, PRKCA, and the DNA fragment D17S21 on chromosome 17q. Hereditas 115: 69-72, 1991.