Orexin Receptor IHC Antibody

Description Orexins are endogeneous peptides that activate Orexin Receptor type 1 and type 2, two closely related G-protein coupled receptors. Orexins-A and -B are hypothalamic peptides derived from a precursor called preproorexin and affect the stimulation of food intake. They act on G protein receptors named orexin receptor-1 (OX1R) and orexin receptor-2 (OX2R), respectively. Stimulation of these receptors by orexins causes an increase in intracellular calcium levels in hypothalamic cells in vitro. In vivo, orexins control feeding and may also be involved in modulating sleep-wake cycles and other hypothalamic functions. Catalog Number IW-PA1100 Quantity 9 ml Host Rabbit Clone Polyclonal Isotype IgG Immunogen A synthetic peptide corresponding to a sequence at the C-terminal of human Orexin Receptor, identical to the related mouse and rat sequence. Purity Immunogen affinity purified Conjugate Unconjugated Species Reactivity Human, mouse, rat. Not tested in other species. Positive Control Brain Cellular Localization Cytoplasmic Form Ready to use solution in PBS with stabilizer and 0.01% sodium azide. No further dilution needed. Serum blocking step should be omitted. Storage Store at 2-8 CC. Do not freeze. Applications IHC-P: Formalin fixed paraffin embedded tissue sections. IHC-Fr: Not tested. ICC: Not tested. Limitations This product is intended for Research Use Only. Interpretation of the test results is solely the responsibility of the user. Precautions Users should follow general laboratory precautions when handling this product. Wear personal protective equipment to avoid contact with skin and eyes. References 1.Blanco, M.; Lopez, M.; Garcia-Caballero, T.; Gallego, R.; Vazquez-Boquete, A.; Morel, G.; Senaris, R.; Casanueva, F.; Dieguez, C.; Beiras, A. : Cellular localization of orexin receptors in human pituitary. J. Clin. Endocr. Metab. 86: 1616-1619, 2001. 2.Sakurai, T.; Amemiya, A.; Ishii, M.; Matsuzaki, I.; Chemelli, R. M.; Tanaka, H.; Williams, S. C.; Richardson, J. A.; Kozlowski, G. P.; Wilson, S.; Arch, J. R. S.; Buckingham, R. E.; Haynes, A. C.; Carr, S. A.; Annan, R. S.; McNulty, D. E.; Liu, W.-S.; Terrett, J. A.; Elshourbagy, N. A.; Bergsma, D. J.; Yanagisawa, M. : Orexins and orexin receptors: a family of hypothalamic neuropeptides and G protein-coupled receptors that regulate feeding behavior. Cell 92: 573-585, 1998.