HSP60 IHC Antibody

Description HSP60 is a member of the chaperonin class of protein factors, which include the Escherichia coli groEL protein and the Rubisco subunit-binding protein of chloroplasts. It acts as a costimulator of human regulatory CD4-positive/CD25 -positive T cells, which inhibit lymphoproliferation and IFNG and TNF secretion by CD4-positive and CD8-positive T cells. HSP60 enhances Treg activity via TLR2, leading to activation of an intracellular signaling cascade that included p38, as well as inhibition of ERK phosphorylation. Suppression of target T cells is mediated by both cell-to-cell contact and by secretion of TGFB and IL10, and it leads to downregulation of ERK, NFKB, and TBET expression. The self-molecule HSP60 can downregulate adaptive immune responses by upregulating Tregs through TLR2 signaling. Catalog Number IW-PA1106 Quantity 9 ml Host Rabbit Clone Polyclonal Isotype IgG Immunogen A synthetic peptide mapping at the middle region of human HSP60, identical to the related rat and mouse sequence. Purity Immunogen affinity purified Conjugate Unconjugated Species Reactivity Human, mouse, rat.Not tested in other species. Positive Control Human intestinal cancer, mammary cancer. Cellular Localization Cytoplasmic Form Ready to use solution in PBS with stabilizer and 0.01% sodium azide. No further dilution needed. Serum blocking step should be omitted. Storage Store at 2-8 CC. Do not freeze. Applications IHC-P: Heat induced epitope retrieval is required on formalin fixed paraffin sections. IHC-Fr: Suitable. ICC: Suitable. Limitations This product is intended for Research Use Only. Interpretation of the test results is solely the responsibility of the user. Precautions Users should follow general laboratory precautions when handling this product. Wear personal protective equipment to avoid contact with skin and eyes. References 1. Cheng, M. Y.; Hartl, F.-U.; Martin, J.; Pollock, R. A.; Kalousek, F.; Neupert, W.; Hallberg, E. M.; Hallberg, R. L.; Horwich, A. L. : Mitochondrial heat-shock protein hsp60 is essential for assembly of proteins imported into yeast mitochondria.Nature 337: 620-625, 1989. 2. Zanin-Zhorov, A.; Cahalon, L.; Tal, G.; Margalit, R.; Lider, O.; Cohen, I. R. : Heat shock protein 60 enhances CD4+CD25+ regulatory T cell function via innate TLR2 signaling. J. Clin. Invest. 116: 2022-2032, 2006.