Cytochrome C IHC Antibody

Description Cytochrome C is located in the mitochondria of all aerobic cells and is involved in the electron transport system. Human cytochrome c has 104 amino acid residues and a molecular weight of 11, 458 and is mapped to 7p15.2. Cytochrome c released from mitochondria has been proposed to be an essential component of an apoptotic pathway responsive to DNA damage and other forms of cell stress. And it has a role in different apoptotic signaling cascades. Catalog Number IW-PA1118 Quantity 9 ml Host Rabbit Clone Polyclonal Isotype IgG Immunogen A synthetic peptide corresponding to a sequence at the C-terminal of human Cytochrome C, identical to the related mouse sequence. Purity Immunogen affinity purified Conjugate Unconjugated Species Reactivity Human, mouse, rat. Not tested in other species. Positive Control Lung, Cellular Localization Cytoplasmic Form Ready to use solution in PBS with stabilizer and 0.01% sodium azide. No further dilution needed. Serum blocking step should be omitted. Storage Store at 2-8 CC. Do not freeze. Applications IHC-P: Heat induced epitope retrieval enhance stain on formalin fixed paraffin sections. IHC-Fr: Formalin or acetone fixed frozen sections. ICC: Not tested. Limitations This product is intended for Research Use Only. Interpretation of the test results is solely the responsibility of the user. Precautions Users should follow general laboratory precautions when handling this product. Wear personal protective equipment to avoid contact with skin and eyes. References 1. Li, K.; Li, Y.; Shelton, J. M.; Richardson, J. A.; Spencer, E.; Chen, Z. J.; Wang, X.; Williams, R. S. : Cytochrome c deficiency causes embryonic lethality and attenuates stress-induced apoptosis. Cell 101: 389-399, 2000.