Anti-Rat CNTF - Biotin

Antibody Details

Product Details

Reactivity Species

Rat

Host Species

Goat

Immunogen

Purified Recombinant Rat CNTF (Accession # P20294.1)

Formulation

This biotinylated antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 50 µg of bovine serum albumin per µg of antibody with no calcium, magnesium, or preservatives present.

Storage and Handling

The lyophilized, biotinylated antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for up to twelve months from date of receipt. The reconstituted biotin conjugate can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted conjugate, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months.

Country of Origin

USA

Shipping

Next Day Ambient

RRID

AB_2829692

Applications and Recommended Usage?
Quality Tested by Leinco

Western Blotting: To detect Rat CNTF this biotin conjugate can be used at a concentration of 0.1 - 0.2 µg/ml. This biotin conjugate should be used in conjunction with compatible second-step reagents such as PN:A106 and a chromogenic substrate such as PN:T343. The detection limit for Rat CNTF is 5 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. A suitable Western blotting control is PN:C329.
ELISA Sandwich Assay: This antibody can be used as the detection antibody in a sandwich ELISA at a concentration of approximately 0.1-0.4 µg/mL when used in conjunction with PN:C1440 as the capture antibody at 1-2 µg/ml and an optimal second step reagent such as PN:A106 for the detection of Rat CNTF.

Other Applications Reported In Literature ?

IHC (NBF/Par.): This antibody should give satisfactory staining results when used at a concentration of 15 µg/ml. The recommended secondary antibody for IHC is PN:A106. For chromogenic detection with high signal and low background use PN:D100 or PN:K107.

Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity

Goat Anti-Rat Ciliary Neurotrophic Factor (CNTF) recognizes Rat CNTF. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research.

Background

Ciliary neurotrophic factor (CNTF) is a cytokine¹ that supports survival and/or differentiation of a variety of neuronal cell types including sensory, sympathetic, and motoneurons.² With these abilities, CNTF has implications for Parkinson's disease and cell-replacement therapies for other disorders.³ CNTF mimics the biological actions of leptin while overcoming "leptin resistance" and appears to reduce inflammatory signaling cascades associated with lipid accumulation in liver and skeletal muscle. The advantages and disadvantages of CNTF has shown promise as a therapeutic strategy to alleviate obesity-associated diseases.⁴

Antigen Details

Protein

CNTF

PubMed

CNTF

NCBI Gene Bank ID

25707

References & Citations

1. Boschero, AC. et al. (2009) Cytokine. 46(1):65-71.

2. Thoenen, H. et al. (1994) J Neurobiol. 25: 1436

3. Hagg, T. et al. (2008) J Neurosci. 28: 2231

4. Febbraio, MA. et al. (2008) J Mol Med. 86: 353

Technical Protocols