Anti-Human FGF-6

Antibody Details

Product Details

Reactivity Species

Human

Host Species

Goat

Immunogen

Purified Recombinant Human FGF-6 (> 98%)

Endotoxin Level

< 10 ng/mg as determined by the LAL method

Formulation

This antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 5.0% w/v trehalose with no calcium, magnesium, or preservatives present.

Storage and Handling

The lyophilized antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for twelve months from date of receipt. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months.

Country of Origin

USA

Shipping

Next Day Ambient

RRID

AB_2830102

Applications and Recommended Usage?
Quality Tested by Leinco

Western Blotting: To detect Human FGF-6 this polyclonal antibody can be used at a concentration of 0.1-0.2 µg/ml. This polyclonal antibody should be used in conjunction with compatible second-step reagents such as PN:G505 and a chromogenic substrate such as PN:T343. The detection limit for Human FGF-6 is 2 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. A suitable Western blotting control is PN:F121.

Other Applications Reported In Literature ?

Neutralization: This antibody is useful for neutralization of Human FGF-6 bioactivity. The antibody dose required to neutralize 50% (ND₅₀) of the biological activity of Human FGF-6 (at 1.0 ng/ml of FGF-6 and 1.0 µg/ml of heparin) is 0.025-0.075 µg/ml.

Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity

Goat Anti-Human Fibroblast Growth Factor 6 (FGF-6) recognizes Human FGF-6. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research.

Background

Fibroblast growth factor 6, also known as FGF6, hst-2 or hst-1-related is a member of the fibroblast growth factor (FGF) family. ^{1, 2} FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. FGF6 is the only known member of the FGF family whose expression is restricted to the muscle cell lineage during development, suggesting it may have a role in myogenesis.³ FGF6 has a dual function in muscle regeneration, stimulating myoblast proliferation/migration and muscle differentiation/hypertrophy.⁴

Antigen Details

Protein

FGF-6

PubMed

FGF-6

NCBI Gene Bank ID

2251

UniProt.org

More Information on Uniprot.org

References & Citations

1. Iida, S. et al. (1992) Oncogene 7: 303

2. Marics, I. et al. (1989) Oncogene 4: 335

3. DeLapeyrière, O. et al. (1996) Exp Cell Res. 224: 143

4. Chanoine, C. et al. (2006) Biochim Biophys Acta 1763: 773

Technical Protocols