Anti-human FGF-5 - Biotin

Antibody Details

Product Details

Reactivity Species

Human

Host Species

Goat

Immunogen

Purified Recombinant Human FGF-5 (> 98%)

Formulation

This biotinylated antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 50 µg of bovine serum albumin per µg of antibody with no calcium, magnesium, or preservatives present.

Storage and Handling

The lyophilized, biotinylated antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for up to twelve months from date of receipt. The reconstituted bioin conjugate can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted conjugate, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months.

Country of Origin

USA

Shipping

Next Day Ambient

RRID

AB_2830138

Applications and Recommended Usage?
Quality Tested by Leinco

Western Blotting: To detect Human FGF-5 this biotin conjugate can be used at a concentration of 0.1 - 0.2 µg/ml. This biotin conjugate should be used in conjunction with compatible second-step reagents such as PN:A106 and a chromogenic substrate such as PN:T343. The detection limit for Human FGF-5 is 5 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. A suitable Western blotting control is PN:F137.

Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity

Goat Anti-Human Fibroblast Growth Factor 5 (FGF-5) recognizes Human FGF-5. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research.

Background

Fibroblast growth factor 5, also known as FGF5 is a secreted signaling protein¹ that is a member of the fibroblast growth factor gene family.² FGF5 is widely expressed in embryonic but scarcely in adult tissues.³ FGF5 contributes to the malignant progression of human astrocytic brain tumours by both autocrine and paracrine effects.³ FGF-5 inhibitors are promising substances against hair loss and/or for promoting hair growth.⁴ FGF5 may also have a functional role in the pathophysiology of age-related macular degeneration (ARMD).⁵

Antigen Details

Protein

FGF-5

PubMed

FGF-5

NCBI Gene Bank ID

2250

UniProt.org

More Information on Uniprot.org

References & Citations

1. Martin, GR. et al. (1997) Cell 78: 1017

2. Thoenen, H. et al. (1994) Eur J Neurosci. 6: 244

3. Berger, W. et al. (2008) Oncogene 27: 4180

4. Suzuki, S. et al. (2003) J Cell Physiol. 197: 272

5. Hjelmeland, LM. et al. (1997) Curr Eye Res. 16: 396

Technical Protocols