AffiPure Normal Human IgG, F(ab’)2 fragment

Antibody Details

Product Details

Host Species

Human

Product Concentration

5.0 - 15 mg/ml

Formulation

This product has been aseptically packaged and formulated in phosphate buffered saline (PBS) pH 7.1-7.5 with no carrier proteins or preservatives.
Product may be provided with carrier proteins or preservatives by request

State of Matter

Sterile Liquid

Storage and Handling

Store at 2-8° C under sterile conditions

Expiration Date

One year after date of receipt

Country of Origin

USA

Shipping

Next Day 2-8°C

RRID

AB_2893724

Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Background

AffiPure™ Normal Human IgG, F(ab')2 fragment antibodies eliminate non-specific binding between Fc portions of antibodies and Fc receptors on cells such as macrophages, dendritic cells, neutrophils, NK cells or B cells. F(ab')2 fragment antibodies are generated by optimal pepsin digestion of the antisera followed by antigen affinity chromatography to eliminate the presence of Fc fragments and whole molecules, as well as leave intact some of the hinge region. F(ab')2 fragments have two antigen-binding F(ab) portions linked by disulfide bonds, and therefore are divalent with a molecular weight of about 110 kDa. F(ab')2 fragments penetrate tissues more efficiently than whole molecules due to their smaller size. This product contains a sufficient quantity of endogenous IgG to block nonspecific binding sites on fixed samples and assay substrates. While most commonly used in flow cytometry this product is useful in other applications such as ELISA, IHC, ICC, Blocking and as a control.