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Anti-Human IgM µ-Chain Specific (Clone HB57) - HRP - 500 µg

ArtNr LEIN-I-1201-500ug
Hersteller Leinco Technologies
Menge 500 ug
Kategorie
Typ Antibody Monoclonal
Applikationen FC, ELISA
Clon HB57
Specific against Human (Homo sapiens)
Host Mouse
Citations 1. Rudich, S. M. et al. (1988) J. Exp. Med. 168:247
ECLASS 10.1 42030590
ECLASS 11.0 42030590
UNSPSC 12352203
Versandbedingung Raumtemperatur
Lieferbar
Manufacturer - Applications
Quality Tested by Leinco FC This antibody has been quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume.
ELISA This antibody is useful as the capture antibody in a sandwich ELISA. The suggested coating concentration is 5 µg/ml (100 µl/well) µg/ml.
Manufacturer - Category
Human Immunoglobulins|Primary Monoclonal Antibodies
Manufacturer - Targets
Human Immunoglobulin, IgM µ-Chain Specific
Country of Origin
USA
Shipping Temperature
Next Day Ambient
Storage Conditions
This conjugated antibody is stable when stored at 2-8°C. Do not freeze.
Cross Reactivity
Cross-reactivity by ELISA against human myeloma proteins:
Human IgG1: 100%
Human IgG2: 100%
Human IgG3: 100%
Human IgG4: 100%
Human IgM: < 0.2%
Human IgA: < 0.1%
Human IgE: < 0.1%
There is no detectable binding to bovine, goat, horse sheep IgG.
NOTE: This Anti-Human IgG, Fc Fragment Specific (Clone HB57) when conjugated to HRP is validated for use in Enzyme Immunoassay for the detection of Human IgG, µ-Chain Specific (Part No.: I-1201)
PubMed
IgM µ-Chain Specific
Manufacturer - Specificity
Anti-Human IgM (µ chain specific) monoclonal antibody reacts with human IgM through an epitope on the heavy chain. This antibody is non-reactive with IgG, IgA or light chains. Clone HB57 produces an antibody with one of the highest affinities available (Ka = 5.34 x 108 M-1).1
RRID
AB_2893815
Concentration
0.5 mg/ml
Formulation
This HRP-conjugated antibody is formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4, 1% BSA. (Warning: Use of sodium azide as a preservative will inhibit the enzyme activity of horseradish peroxidase)
Antigen Distribution
Surface IgM is expressed on B-lymphocytes.
Additional Information
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.
Keywords
Monoclonal Antibody, IgM µ-Chain Specific, HB57, Human IgM gamma chain specific, Anti-Human IgM µ-Chain Specific Purified, Clone HB57, HRP, ELISA

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

Menge: 500 ug
Lieferbar: In stock
lieferbar

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