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Anti-Mouse IL-7 Rα - Biotin - 50 µg

Anti-Mouse IL-7 Rα - Biotin - 50 µg

ArtNr	LEIN-I-795-50ug
Hersteller	Leinco Technologies
Menge	50 ug
Kategorie	Antibodies Flow Cytometry Western Blot Primary Antibodies
Typ	Antibody Primary
Applikationen	WB, FC
Specific against	Mouse (Murine, Mus musculus)
Host	Caprine
Citations	1. Fry, TJ. et al. (2005) J Immunol. 174: 6571 2. Murre, C. et al. (2002) Embo J. 21: 103
NCBI	16197
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Versandbedingung	Raumtemperatur
Lieferbar
Manufacturer - Applications	Quality Tested by Leinco Flow Cytometry: It is recommended to use the indirect method for signal enhancement when enumerating cells expressing IL-7 Rα. A suggested method would be to stain cells expressing IL-7 Rα with 0.25 µg per 1 x 10⁶ cells in a total staining volume of 100 µl followed by PN:A104. Western Blotting: To detect Mouse IL-7 Rα this biotin conjugate can be used at a concentration of 0.1 - 0.2 µg/ml. This biotin conjugate should be used in conjunction with compatible second-step reagents such as PN:A106 and a chromogenic substrate such as PN:T343. The detection limit for Mouse IL-7 Rα is 5 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. A suitable Western blotting control is PN:I-581.
Manufacturer - Category	Polyclonal Antibodies>Biotin Growth Factors & Cytokines
Manufacturer - Targets	IL-7 Rα
Country of Origin	USA
Shipping Temperature	Next Day Ambient
Storage Conditions	The lyophilized, biotinylated antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for up to twelve months from date of receipt. The reconstituted biotin conjugate can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted conjugate, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months.
Product Description	CD127 (IL7R-α) is a 60-90 kD type I cytokine receptor involved in the regulation of lymphopoiesis. The active receptor is an α/γ chain heterodimer. The common γ chain, which also associates with the IL-2 receptor, serves mainly to activate signal transduction via the IL7R complex. CD127 also interacts with the Thymic Stromal Lymphopoietin Receptor (TSLPR). CD127 determines signaling events via its association with cytoplasmic signaling molecules and has been reported to be a useful marker for identifying memory and effector T cells.
Background	CD127 (IL7R-α) is a 60-90 kD type I cytokine receptor involved in the regulation of lymphopoiesis. The active receptor is an α/γ chain heterodimer. The common γ chain, which also associates with the IL-2 receptor, serves mainly to activate signal transduction via the IL7R complex. CD127 also interacts with the Thymic Stromal Lymphopoietin Receptor (TSLPR). CD127 determines signaling events via its association with cytoplasmic signaling molecules and has been reported to be a useful marker for identifying memory and effector T cells.
PubMed	IL-7 Rα
Manufacturer - Specificity	Goat Anti-Mouse Interleukin 7 Receptor Alpha (IL-7 Rα) recognizes Mouse IL-7 Rα. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research.
RRID	AB_2830916
Formulation	This biotinylated antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 50 µg of bovine serum albumin per µg of antibody with no calcium, magnesium, or preservatives present.
Antigen Distribution	IL-7Rα is expressed on bone marrow lymphoid precursors, pro-B cells, monocytes/macrophages, NK-cells, naïve T cells, memory CD4+ and CD8+ T cells, intestinal colorectal and renal carcinoma cell lines, and keratinocytes.
Immunogen	Purified Recombinant Mouse IL-7 Rα (Accession # P16872)
Additional Information	Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.
Keywords	Polyclonal Antibody, Interleukin-7 Receptor Alpha, IL7R, CD127, CDW127, IL-7 Rα, Mouse, Anti-Mouse IL-7 Rα-Biotin, Mouse IL 7 Rα, IL 7 Rα, CD127 antigen, CD127, CD127ILRA, CDW127, IL-7 R alpha, IL-7 receptor subunit alpha, IL7R alpha, IL-7R subunit alpha, IL7R, IL7RA, IL-7Ra, IL-7R-alpha, interleukin 7 receptor alpha chain, interleukin 7 receptor isoform H5-6, interleukin 7 receptor, interleukin-7 receptor subunit alpha

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LEIN-I-795-50ug-safety-datasheet.pdf