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Anti-Human CD137L (4-1BBL) -Biotin

ArtNr LEIN-L248-50ug
Hersteller Leinco Technologies
Menge 50 ug
Kategorie
Typ Antibody Primary
Applikationen WB, FC
Specific against Human (Homo sapiens)
Host Caprine
NCBI 8744
ECLASS 10.1 32160702
ECLASS 11.0 32160702
UNSPSC 12352203
Versandbedingung Raumtemperatur
Lieferbar
Manufacturer - Applications
Quality Tested by Leinco Western Blotting: To detect Human 4-1BB Ligand this biotin conjugate can be used at a concentration of 0.1-0.2 µg/ml. This biotin conjugate should be used in conjunction with compatible second-step reagents such as PN:A106 and a chromogenic substrate such as PN:T343. The detection limit for Human 4-1BB Ligand is 2 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. A suitable Western blotting control is PN:F131.
Flow Cytometry: Suitable for use at concentration 2.5 µg/106 cells
Manufacturer - Category
Polyclonal Antibodies>Biotin Growth Factors & Cytokines|Primary Monoclonal Antibodies>Immune Checkpoints
Manufacturer - Targets
4-1BBL
Country of Origin
USA
Shipping Temperature
Next Day Ambient
Storage Conditions
The lyophilized, biotinylated antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for up to twelve months from date of receipt. The reconstituted biotin conjugate can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted conjugate, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months.
PubMed
4-1BBL
Manufacturer - Specificity
Goat Anti-Human 4-1BB Ligand recognizes Human 4-1BB Ligand. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research.
RRID
AB_2831168
Formulation
This biotinylated antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 50 µg of bovine serum albumin per µg of antibody with no calcium, magnesium, or preservatives present.
Immunogen
Purified Recombinant Human 4-1BB Ligand (aa 71-254) (Accession # P41273)
Additional Information
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

Menge: 50 ug
Lieferbar: In stock
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