Antibody Details
Product Details
Reactivity Species
Mouse
Host Species
Goat
Immunogen
Purified Mouse IgG, Fc (Gamma) Fragment (> 98%)
Product Concentration
1.4 mg/ml
Formulation
This secondary antibody is lyophilized from 0.01M phosphate buffered saline (PBS) pH 7.6 containing 0.25M NaCl, 15 mg/ml IgG free BSA and 0.05% sodium azide as a preservative.
Product Preparation
This F(ab')2 secondary antibody was generated by optimal pepsin degestion of the antisera followed by antigen affinity chromatography to elimiate the presence of Fc fragment and whole molecules.
Storage and Handling
This lyophilized secondary antibody conjugate should be stored desiccated at 2-8°C. The reconstituted Rhodamine RED X conjugate containing glycerol should be stored at -20°C in a manual defrost freezer.
Expiration Date
One year from date of reconstitution
Country of Origin
USA
Shipping
Next Day Ambient
RRID
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.
Description
Specificity
Goat F(ab')2 Anti-Mouse IgG, Fc (Gamma) Fragment recognizes Mouse IgG, Fc (Gamma) Fragment. This secondary antibody was purified using antigen affinity chromatography and adsorbed against serum proteins and immunoglobulins to minimize species cross-reactivity.
Antigen Details
Protein
Fc (Gamma) Fragment
PubMed
Fc (Gamma) Fragment
References & Citations
1. Negishi, M. et al. (1998) Biochem J. 335(Pt 1):35-42. PubMed
Technical Protocols