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Anti-Mouse CCL1-Biotin

Anti-Mouse CCL1-Biotin

ArtNr	LEIN-T504-50ug
Hersteller	Leinco Technologies
Menge	50 ug
Kategorie	Antibodies Primary Antibodies
Typ	Antibody Primary
Applikationen	WB, ELISA
Specific against	Mouse (Murine, Mus musculus)
Host	Caprine
Citations	1. Krangel, MS. et al. (1992) Proceedings of the National Acad of Sci. 89: 2950 2. Roos, RS. et al. (1997) J Biol Chem. 272: 17251 3. Teran, LM et al. (2006) Eur Respir J. 28: 59
NCBI	20290
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Versandbedingung	Raumtemperatur
Lieferbar
Manufacturer - Applications	Quality Tested by Leinco Western Blotting: To detect Mouse CCL1 this biotin conjugate can be used at a concentration of 0.1 µg/ml. This biotin conjugate should be used in conjunction with compatible second-step reagents such as PN:A106 and a chromogenic substrate such as PN:T343. The detection limit for Mouse CCL1 is 2 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. A suitable Western blotting control is PN:T410. ELISA Sandwich Assay: This antibody can be used as the detection antibody in a sandwich ELISA at a concentration of approximately 0.1-0.4 µg/ml when used in conjunction with PN:T459 as the capture antibody at 2-8 µg/ml and an optimal second step reagent such as PN:A106 for the detection of Mouse CCL1.
Manufacturer - Category	Polyclonal Antibodies>Biotin Growth Factors & Cytokines
Manufacturer - Targets	CCL1
Country of Origin	USA
Shipping Temperature	Next Day Ambient
Storage Conditions	The lyophilized, biotinylated antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for up to twelve months from date of receipt. The reconstituted biotin conjugate can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted conjugate, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months.
Product Description	Chemokine (C-C motif) ligand 1 (CCL1) is a small glycoprotein secreted by activated T cells that belongs to a family inflammatory cytokines known as chemokines.1 CCL1 attracts monocytes, NK cells, and immature B cells and dendritic cells by interacting with a cell surface chemokine receptor called CCR8.2 CCL1 may play a role in lymphocyte recruitment in bronchial asthma.3
Background	Chemokine (C-C motif) ligand 1 (CCL1) is a small glycoprotein secreted by activated T cells that belongs to a family inflammatory cytokines known as chemokines.¹ CCL1 attracts monocytes, NK cells, and immature B cells and dendritic cells by interacting with a cell surface chemokine receptor called CCR8.² CCL1 may play a role in lymphocyte recruitment in bronchial asthma.³
PubMed	CCL1
Manufacturer - Specificity	Goat Anti-Mouse Chemokine (C-C Motif) Ligand 1 (CCL1) recognizes Mouse CCL1. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research.
RRID	AB_2832004
Formulation	This biotinylated antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.4 containing 50 µg of bovine serum albumin per µg of antibody with no calcium, magnesium, or preservatives present.
Immunogen	Purified Recombinant Mouse CCL1 (Accession # P10146)
Additional Information	Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

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LEIN-T504-50ug-safety-datasheet.pdf