Actin alpha 2 Antibody

Blue Ice

98 kDa

The alpha-actinins are a multigene family of four actin-binding proteins related to dystrophin. The two skeletal muscle isoforms of alpha-actinin (ACTN2 and ACTN3) are major structural components of the Z-line involved in anchoring the actin-containing thin filaments. In humans, ACTN2 is expressed in all muscle fibres, while ACTN3 expression is restricted to a subset of type 2 fibres. Murine Actn2 and Actn3 are differentially expressed, spatially and temporally, during embryonic development and, in contrast to humans, alpha-actinin-2 expression does not completely overlap alpha-actinin-3 in postnatal skeletal muscle, suggesting independent function.

None

Antibody is purified by protein A chromatography method.

batch dependent

Actin alpha 2 antibody can be used for detection of Actin alpha 2 by ELISA at 1:312500. Actin alpha 2 antibody can be used for detection of Actin alpha 2 by western blot at 0.625 μ g/mL, and HRP conjugated secondary antibody should be diluted 1:50, 000 - 100, 000.

Polyclonal

Mus musculus

Optimal dilutions for each application to be determined by the researcher.