SFPQ Antibody

Blue Ice

78 kDa

SFPQ is DNA- and RNA binding protein. It is essential pre-mRNA splicing factor required early in spliceosome formation and for splicing catalytic step II, probably as an heteromer with NONO. It binds to pre-mRNA in spliceosome C complex, and specifically binds to intronic polypyrimidine tracts. It interacts with U5 snRNA. It may be involved in a pre-mRNA coupled splicing and polyadenylation process as component of a snRNP-free complex with SNRPA/U1A. SFPQ may be involved in homologous DNA pairing. SFPQ is involved in transcriptional regulation. It binds the DNA sequence 5'-CTGAGTC-3' in the insulin-like growth factor response element (IGFRE) and inhibits IGF-I-stimulated transcriptional activity.

None

Antibody is purified by protein A chromatography method.

batch dependent

SFPQ antibody can be used for detection of SFPQ by ELISA at 1:1562500. SFPQ antibody can be used for detection of SFPQ by western blot at 5.0 μ g/mL, and HRP conjugated secondary antibody should be diluted 1:50, 000 - 100, 000.

Polyclonal

Homo sapiens

Optimal dilutions for each application to be determined by the researcher.