CD10 Antibody [2A1H5E1]

IHC(P):Dilution 1:100 - 1:200
IHC(F):Dilution 1:200 - 1:400
ELISA:Propose dilution 1:10, 000.
Determining optimal working dilutions by titration test.

CD10 monoclonal antibody can be stored at -20°C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

CD10 is a 100 kDa glycoprotein, also designated Common Acute Lymphocytic Leukemia Antigen (CALLA). It is a cell surface enzyme with neutral metalloendopeptidase activity which inactivates a variety of biologically active peptides. CD10 is expressed on the cells of lymphoblastic, Burkitt’s, and follicular germinal center lymphomas, and on cells from patients with chronic myelocytic leukemia (CML). It is also expressed on the surface of normal early lymphoid progenitor cells, immature B cells within adult bone marrow and germinal center B cells within lymphoid tissue. CD10 is also present on breast myoepithelial cells, bile canaliculi, fibroblasts, with especially high expression on the brush border of kidney and gut epithelial cells.

Antibody is purified by protein G affinity chromatography.

Ni-NTA purified recombinant human CD-10 expressed in E. Coli strain BL21 (DE3).

membrane metallo-endopeptidase

Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.