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TGF alpha Antibody [MF9]

ArtNr PRS-33-211-100ug
Hersteller ProSci
Menge 100 ug
Kategorie
Typ Antibody Monoclonal
Format Liquid
Applikationen WB, FC, IF, IHC
Clon MF9
Specific against Human (Homo sapiens), Mouse (Murine, Mus musculus), Rat (Rattus norvegicus), Cattle (Bovine)
Host Mouse
Isotype IgG1 Kappa
Konjugat/Tag Unconjugated
NCBI TGFA
ECLASS 10.1 42030590
ECLASS 11.0 42030590
UNSPSC 12352203
Alias TFGAProtransforming growth factor alpha, EGF-like TGF, TGF-alpha
Lieferbar
Manufacturer - Applications
Western blot: 1-2 ug/ml

Flow Cytometry: 0.5-1 ug/million cells

IF: 1-2 ug/ml

IHC (FFPE): 1-2 ug/ml for 30 min at RT (1)

The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the TGF alpha antibody to be titered up or down for optimal performance.

1. Staining of FFPE tissues requires boiling sections in 10mM Tris with 1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 min.

Shipping Temperature
blue ice or room temperature
Storage Conditions
Aliquot and Store at 2-8°C. Avoid freez-thaw cycles.
Manufacturer - Research Area
Growth Factor, Antibody Pairs, Functional Assays
Background
TGF alpha is a growth factor with 33% homology to EGF, binds to EGFR, activates tyrosine phosphorylation of the receptor, and stimulates cell proliferation. It plays a role in tumor initiation by inducing the reversible transformed phenotype. This antibody reacts with the C-terminus of TGF alpha and shows no cross-reaction with EGF and the neuropeptide synenkephalin. Staining with the antibody is completely blocked by the peptide used for raising this antibody.
Purification
Protein G affinity chromatography
Concentration
0.2 mg/mL
Buffer
PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Immunogen
A 17-amino acid synthetic peptide from the carboxyl-terminus of rat TGF alpha was used as the immunogen for this antibody.
Clonality
Monoclonal
NCBI Official Name
transforming growth factor, alpha
NCBI Organism
Homo sapiens
Disclaimer
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

Hinweis: Die dargestellten Informationen und Dokumente (Bedienungsanleitung, Produktdatenblatt, Sicherheitsdatenblatt und Analysezertifikat) entsprechen unserem letzten Update und sollten lediglich der Orientierung dienen. Wir übernehmen keine Garantie für die Aktualität. Für spezifische Anforderungen bitten wir Sie, uns eine Anfrage zu stellen.

Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

Menge: 100 ug
Lieferbar: In stock
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