Lambda Light Chain Antibody [LLC/1738]

Flow Cytometry: 0.5-1 ug/million cells in 0.1ml

Immunofluorescence: 1-2 ug/ml

Western blot: 0.5-1 ug/ml

Immunohistochemistry (FFPE): 0.5-1 ug/ml for 30 minutes at RT

The optimal dilution of the Lambda Light Chain antibody for each application should be determined by the researcher.

Aliquot and Store at 2-8°C. Avoid freez-thaw cycles.

This mAb is specific to lambda light chain of immunoglobulin and shows no cross-reaction with kappa light chain or any of the five heavy chains. In mammals, the two light chains in an antibody are always identical, with only one type of light chain, kappa or lambda. The ratio of Kappa to Lambda is 70:30. However, with the occurrence of multiple myeloma or other B-cell malignancies this ratio is disturbed. Antibody to the lambda light chain is reportedly useful in the identification of leukemias, plasmacytomas, and certain non-Hodgkin's lymphomas. Demonstration of clonality in lymphoid infiltrates indicates that the infiltrate is malignant.

0.2 mg/mL

Purified human lambda light chain was used as the immunogen for this Lambda Light Chain antibody.

immunoglobulin lambda variable 1-51

Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.