Filaggrin Antibody [FLG/1562]

Primary Antibodies

Aliquot and Store at 2-8˚ C. Avoid freez-thaw cycles.

Filaggrin (FLG) is an intermediate filament-associated protein that aggregates keratin intermediate filaments in mammalian epidermis. It is initially synthesized as a polyprotein precursor, profilaggrin (consisting of multiple Filaggrin units of 324 aa each), which is localized in keratohyalin granules, and is subsequently proteolytically processed into individual functional Filaggrin molecules. Active Filaggrin is present at a level of the epidermis where keratinocytes are in transition between the live nucleated granular layer and the anucleate cornified layer, suggesting that filaggrin aids in the terminal differentiation process by facilitating apoptotic machinery.

None

0.2 mg/mL

Flow Cytometry: 0.5-1ug/10^6 cells in 0.1ml
IF: 1-2 ug/ml
IHC (FFPE): 0.5-1 ug/ml for 30 min at RT
Optimal dilution of the Filaggrin antibody should be determined by the researcher.

Monoclonal

homo sapiens

Optimal dilutions for each application to be determined by the researcher