Erythropoietin Antibody [EPO/1368]

Primary Antibodies

Aliquot and Store at 2-8˚ C. Avoid freez-thaw cycles.

Erythropoietin / EPO is a secreted, glycosylated cytokine hormone composed of four alpha helical bundles. It is the primary factor responsible for regulating erythropoiesis during steady-state conditions and in response to blood loss and hemorrhage in the adult organism. Erythropoietin is synthesized by the kidney and stimulates the proliferation and maturation of bone marrow erythroid precursor cells. The protein is found in the plasma and regulates red cell production by promoting erythroid differentiation and initiating hemoglobin synthesis.

None

0.2 mg/mL

WB: 0.5-1 ug/ml
IF: 1-2 ug/ml
Immunohistochemistry (FFPE): 4-8 ug/ml for 30 min at RT (1)
Flow Cytometry: 0.5-1 ug/million cells in 0.1ml
Titering of the EPO antibody may be required for optimal performance.

1. Required HIER: boil tissue sections in 10mM citrate buffer, pH 6, for 10-20 min.

Monoclonal

Homo sapiens

Optimal dilutions for each application to be determined by the researcher