Transferrin Receptor (Extracellular domain) Antibody [TFRC/1817]

Primary Antibodies

Aliquot and Store at 2-8˚ C. Avoid freez-thaw cycles.

It recognizes a ~85-95kDa protein which is identified as cell surface transferrin receptor (CD71), a disulfide-bonded homodimeric glycoprotein of 180-190kDa. This mAb is highly specific to CD71 and shows no cross-reaction with other related proteins. Ligand for transferrin receptor is the serum iron transport protein, transferrin. This receptor is broadly distributed in carcinomas, sarcomas, leukemias, and lymphomas. CD71/Transferrin receptor has been reported to be associated with cell proliferation in both normal and neoplastic tissues and useful in predicting clinical behavior or response to therapy in a number of malignancies including breast cancer.

None

0.2 mg/mL

WB: 0.5-1 ug/ml
ELISA: 2-4 ug/ml; order the BSA free formulation for coating
Flow Cytometry: 0.5-1 ug/million cells in 0.1ml
Immunofluorescence: 1-2 ug/ml
IHC (FFPE): 1-2 ug/ml for 30 min at RT
Optimal dilution of the CD71 antibody should be determined by the researcher.

Monoclonal

Homo sapiens

Optimal dilutions for each application to be determined by the researcher