p53 Antibody [CTA53-1]

Immunofluorescence: 1-2 ug/ml

Western blot: 1-2 ug/ml

IHC (FFPE): 1-2 ug/ml for 30 minutes at RT (1)

Prediluted format : incubate for 30 min at RT (2)

Titering of the p53 antibody may be required for optimal performance.

1. FFPE testing requires sections to be boiled in pH6 10mM citrate buffer for 10-20 minutes, followed by cooling at RT for 20 minutes, prior to staining.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Aliquot and Store at 2-8°C. Avoid freez-thaw cycles.

This gene encodes a tumor suppressor protein containing transcriptional activation, DNA binding, and oligomerization domains. The encoded protein responds to diverse cellular stresses to regulate expression of target genes, thereby inducing cell cycle arrest, apoptosis, senescence, DNA repair, or changes in metabolism. Mutations in this gene are associated with a variety of human cancers, including hereditary cancers such as Li-Fraumeni syndrome. Alternative splicing of this gene and the use of alternate promoters result in multiple transcript variants and isoforms. Additional isoforms have also been shown to result from the use of alternate translation initiation codons. [RefSeq]

0.2 mg/mL

Human recombinant protein was used as the immunogen for this p53 antibody.

tumor protein p53

Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.