Mitochondrial Antibody [MTL56]

Primary Antibodies

Aliquot and Store at 2-8˚ C. Avoid freez-thaw cycles.

The mitochondrial marker antibody is specific for an antigen associated with the mitochondria in human cells. It can be used to stain mitochondria in cell or tissue preparations and as a mitochondrial marker in subcellular fractions.

None

0.2 mg/mL

IHC (FFPE): 0.5-1 ug/ml for 30 minutes at RT (1)
Prediluted format : incubate for 30 min at RT (2)
IF: 0.5-1 ug/ml
Western blot: 1-2 ug/ml
Titering of the Mitochondrial antibody may be required for optimal performance.1. FFPE staining is enhanced by boiling tissue sections in 10mM citrate buffer, pH 6, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Monoclonal

Optimal dilutions for each application to be determined by the researcher