IgM Antibody [MuHC2]

ArtNr	PRS-33-888-100ug
Hersteller	ProSci
Menge	100 ug
Kategorie	Oncology Neuroscience Protein Aggregation, Clearance & Misfolding Neural Development Antibodies By Cell Type Blastoma Carcinoma Hematology Leukemia Lymphoma Myeloma Melanoma Sarcoma Cell Biology Cell Movement Cell Adhesion Cell Culture Cytokines Adenocarcinoma Immunohistochemistry Western Blot Immunofluorescence Secondary Antibodies By Organ Bladder Cancer Colorectal Cancer Heart & Blood Cancer
Typ	Antibody Monoclonal
Format	Liquid
Applikationen	WB, IF, IHC
Clon	MuHC2
Specific against	Human (Homo sapiens)
Host	Mouse
Isotype	IgG1 Kappa
Konjugat/Tag	Unconjugated
NCBI	IGHM
ECLASS 10.1	42030590
ECLASS 11.0	42030590
UNSPSC	12352203
Versandbedingung	Gekühlt
Lieferbar
Manufacturer - Type	Primary Antibodies
Shipping Temperature	Blue Ice
Storage Conditions	Aliquot and Store at 2-8˚ C. Avoid freez-thaw cycles.
Manufacturer - Research Area	Immunology
Background	IgM is the first antibody generated in an immune response to an antigen. It is generally a pentamer with each of the five immunoglobulins linked together with disulfide bonds. In its pentamer form, it has a molecular mass of 970 kDa and 10 antigen binding sites (due to the large size of most antigens, not all binding sites can be filled simultaneously. IgM antibodies account for approximately 5%-10% of all the antibody in the body.
By Type	Immunology
Modifications	None
Purification	Protein G
Concentration	0.2 mg/mL
Buffer	PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Application Note	Immunofluorescence: 0.5-1 ug/ml Western blot: 0.5-1 ug/ml IHC (FFPE): 0.5-1 ug/ml for 30 minutes at RT (1) Prediluted format : incubate for 30 min at RT (2) Due to differences in protocols and secondaries, the IgM antibody may need to be titered for optimal performance. 1. FFPE staining requires boiling sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes. 2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Immunogen	Human Mu heavy chain was used as the immunogen for this IgM antibody.
Clonality	Monoclonal
NCBI Official Name	immunoglobulin heavy constant mu
NCBI Organism	homo sapiens
Disclaimer	Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
User Note	Optimal dilutions for each application to be determined by the researcher

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

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Menge: 100 ug

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Lieferung vsl. bis 06.11.2025

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