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Nucleolin Antibody [NPC23-2]

Nucleolin Antibody [NPC23-2]

ArtNr	PRS-34-019-100ug
Hersteller	ProSci
Menge	100 ug
Kategorie	Oncology Neuroscience Molecular Targets for Neuroscience Antibodies By Cell Type Blastoma Carcinoma Flow Cytometry Immunohistochemistry Western Blot Immunofluorescence Primary Antibodies By Organ Renal Cancer
Typ	Antibody Monoclonal
Format	Liquid
Applikationen	WB, FC, IF, IHC-P
Clon	NPC23-2
Specific against	Human (Homo sapiens), Mouse (Murine, Mus musculus)
Host	Mouse
Isotype	IgG1
Konjugat/Tag	Unconjugated
NCBI	NCL
ECLASS 10.1	42030590
ECLASS 11.0	42030590
UNSPSC	12352203
Alias	Nucleolin, Protein C23, NCL
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Manufacturer - Applications	IF: 1-2 ug/ml Flow Cytometry: 0.5-1 ug/million cells in 0.1ml Western blot: 1-2 ug/ml Immunohistochemistry (FFPE): 0.5-1 ug/ml for 30 min at RT (1) Prediluted format: incubate for 30 min at RT (2) Titering of the Nucleolin antibody may be required for optimal performance. 1. FFPE testing requires sections to be boiled in pH6 10mM citrate buffer for 10-20 minutes, followed by cooling at RT for 20 minutes, prior to staining. 2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Shipping Temperature	blue ice or room temperature
Storage Conditions	Aliquot and Store at 2-8°C. Avoid freez-thaw cycles.
Manufacturer - Research Area	Other
Background	Nucleolin (NCL) is the major nucleolar phosphoprotein of growing eukaryotic cells. It is located mainly in dense fibrillar regions of the nucleolus. NCL is found associated with intranucleolar chromatin and pre-ribosomal particles. It induces chromatin decondensation by binding to histone H1. It is thought to play a role in pre-rRNA transcription and ribosome assembly.
Purification	Protein G affinity chromatography
Concentration	0.2 mg/mL
Buffer	PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Immunogen	Recombinant human protein was used as the immunogen for the Nucleolin antibody.
Clonality	Monoclonal
NCBI Official Name	nucleolin
NCBI Organism	Homo sapiens
Disclaimer	Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

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Menge: 100 ug

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Lieferung vsl. bis 02.10.2025

Express-Lieferung: 1 Tage sparen

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