CD35 Antibody [SPM554]

Primary Antibodies

Aliquot and Store at 2-8˚ C. Avoid freez-thaw cycles.

Recognizes a protein of 210-220kDa, which is identified as the Complement receptor 1 or CD35. This mAb is specific for a site in CR1 that is distal from the C3b-binding site, so that it is unable to block CR1/CD35 activity. It is highly specific and shows no cross-reaction with CR2/CD21. The primary function of CR1 is to serve as the cellular receptor for C3b and C4b, the most important components of the complement system leading to clearance of foreign macromolecules. The Knops blood group system is a system of antigens located on this protein. Follicular dendritic cells (FDC) are restricted to the B-cell regions of secondary lymphoid follicles. They are CD21+/CD35+/CD1a-. This mAb labels follicular dendritic cells and follicular dendritic cell sarcoma.

None

0.2 mg/mL

Flow Cytometry: 0.5-1 ug/million cells in 0.1ml
Immunofluorescence: 1-2 ug/ml
Immunohistochemistry (FFPE): 0.5-1 ug/ml for 30 minutes at RT (1)
Prediluted format: incubate for 30 min at RT (2)
The optimal dilution of the CD35 antibody for each application should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Monoclonal

Homo sapiens

Optimal dilutions for each application to be determined by the researcher