Vergleich

Alpha Fetoprotein Antibody [SPM334]

ArtNr PRS-34-102-100ug
Hersteller ProSci
Menge 100 ug
Kategorie
Typ Antibody Monoclonal
Format Liquid
Applikationen FC, IF, IHC
Clon SPM334
Specific against Human (Homo sapiens), Monkey (Cynomolgus, Simian), Dog (Canine, Canis lupus familiaris), Pig (Porcine, Sus scrofa domesticus)
Host Mouse
Isotype IgG2a Kappa
Konjugat/Tag Unconjugated
NCBI AFP
ECLASS 10.1 42030590
ECLASS 11.0 42030590
UNSPSC 12352203
Alias AFP Antibody: FETA,HPAFP,Alpha-fetoprotein,Alpha-1-fetoprotein
Versandbedingung Gekühlt
Lieferbar
Manufacturer - Type
Primary Antibodies
Shipping Temperature
Blue Ice
Storage Conditions
Aliquot and Store at 2-8˚ C. Avoid freez-thaw cycles.
Manufacturer - Research Area
Homeostasis
Background
It recognizes an oncofetal glycoprotein with a single chain of 70kDa, which is identified as alpha fetoprotein (AFP). This mAb is highly specific to AFP and shows no cross-reaction with other oncofetal antigens or serum albumin. AFP is normally synthesized in the liver, intestinal tract, and yolk sac of the fetus. Antibody to AFP has been shown to be useful in detecting hepatocellular carcinomas (HCC) and germ cell neoplasms, especially yolk sac tumors.
By Type
Homeostasis
Modifications
None
Purification
Protein G affinity chromatography
Concentration
0.2 mg/mL
Buffer
PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Application Note
Flow Cytometry: 0.5-1 ug/million cells in 0.1ml
Immunofluorescence: 0.5-1 ug/ml
Immunohistochemistry (FFPE): 1-2 ug/ml for 30 minutes at RT (1)
Prediluted format : incubate for 30 min at RT (2)
The optimal dilution of the Alpha Fetoprotein antibody for each application should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Manufacturer - Specificity
Does not react with Cow, Cat, Mouse, Rat.
Immunogen
Alpha fetoprotein purified from serum of a hepatoma patient was used as the immunogen for this AFP antibody.
Clonality
Monoclonal
NCBI Official Name
alpha-fetoprotein
NCBI Organism
Homo sapiens
Disclaimer
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
User Note
Optimal dilutions for each application to be determined by the researcher

Hinweis: Die dargestellten Informationen und Dokumente (Bedienungsanleitung, Produktdatenblatt, Sicherheitsdatenblatt und Analysezertifikat) entsprechen unserem letzten Update und sollten lediglich der Orientierung dienen. Wir übernehmen keine Garantie für die Aktualität. Für spezifische Anforderungen bitten wir Sie, uns eine Anfrage zu stellen.

Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

Menge: 100 ug
Lieferbar: In stock
lieferbar

Vergleichen

Auf den Wunschzettel

Angebot anfordern

Lieferzeit anfragen

Technische Frage stellen

Bulk-Anfrage stellen

Fragen zum Produkt?
 
Schließen