Fas Antibody [APO-1-3] (preservative free)

ELISA: (direct or indirect: 1:2, 000-1:10, 000). Western blot: (1:2, 000-1:5, 000 using ECL. Suggested blocking and dilution buffer is TBST with 0.05% Tween 20 and 5% skim milk. Suggested incubation time is 1 hour at rooom temperature). Optimal conditions should be determined individually for each application.

Stable for at least 1 year after receipt when stored at -20°C.

Fas (CD95) is a member of the death receptor (DR) family, a subfamily of the tumor necrosis factor receptor superfamily. The formation of the Fas death-inducing signaling complex (DISC) is the initial step of Fas signaling. Activation of procaspase-8 at the DISC leads to the induction of DR-mediated apoptosis. Stimulation of Fas has been also reported to trigger non-apoptotic pathways. It has been shown that membrane-bound FasL is essential for the cytotoxic activity, whereas soluble FasL appears to promote autoimmunity and tumorigenesis via induction of non-apoptotic pathways, in particular NF-kappaB.

>95% (SDS-PAGE)

Liquid. In PBS. Preservative free formulation.

Monoclonal

Homo sapiens