Nampt Antibody

ELISA: (direct and indirect: 1:2, 000-1:5, 000). Immunohistochemistry: (paraffin sections, 10ug/ml). Western Blot: (1:2, 000-1:5, 000 using ECL; suggested blocking and dilution buffer is PBST containing 0.05% Tween 20 and 5% skim milk; suggested incubation time is 1 hour at room temperature). Optimal conditions must be determined individually for each application.

Stable for at least 6 months after receipt when stored at -20°C.

Nicotinamide phosphoribosyltransferase (Nampt; pre-B cell colony-enhancing factor; PBEF; Visfatin) is an 52kDa adipokine secreted by adipose tissue and involved in the biosynthesis of nicotinamide adenine dinucleotide (NAD+). Two forms of Nampt exist, an intracellular form (iNampt) and an extracellular form (eNampt). While the function of iNampt as an essential and rate-limiting NAD+ biosynthetic enzyme is well established, the physiological role of eNampt is still a matter of debate. Nampt has various functions, including the promotion of vascular smooth muscle cell maturation and inhibition of neutrophil apoptosis. It activates insulin receptor and has insulin-mimetic effects, lowering blood glucose and improving insulin sensitivity. The protein is highly expressed in visceral fat and serum levels of the protein correlate with obesity.

1 mg/ml

Synthetic peptide corresponding to aa 413-431 (V413FKDPVADPNKRSKKGRLS431) of human Nampt (Visfatin/PBEF).

nicotinamide phosphoribosyltransferase

Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.