Stearoyl-CoA Desaturase-1 Antibody

ELISA: (direct and indirect: 1:2, 000-1:5, 000). Western Blot: (1:2, 000-1:5, 000 using ECL; suggested blocking and dilution buffer is PBST containing 0.05% Tween 20 and 5% skim milk; suggested incubation time is 1 hour at room temperature). Optimal conditions must be determined individually for each application.

Stable for at least 6 months after receipt when stored at -20°C.

Stearoyl-CoA desaturase (SCD) is a regulatory enzyme in lipogenesis. The enzyme is constitutively expressed in adipose tissue and is induced in liver in response to fat free, high carbohydrate diet. Several SCD isoforms (SCD1, -2, and -3) exist in the mouse. Mice with a targeted disruption of the SCD1 isoform had reduced body adiposity, increased insulin sensitivity, and resistance to diet-induced weight gain. The protection from obesity involved increased energy expenditure and increased oxygen consumption. SCD1 deficiency shows activation of lipid oxidation in addition to reduced triglyceride synthesis and storage.

1 mg/ml

Synthetic peptide corresponding to aa 21-33 (A21PPSGNEREKVKT33) of mouse SCD1.

stearoyl-Coenzyme A desaturase 1

Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.