Artemin Antibody

Primary Antibodies, Antibody Pairs

Artemin antibody is stable for at least 2 years from date of receipt at -20˚ C. The reconstituted antibody is stable for at least two weeks at 2-8˚ C. Frozen aliquots are stable for at least 6 months when stored at -20˚ C. Avoid repeated freeze-thaw cycles.

Artemin has been shown in culture to support the survival of a number of peripheral neuron populations and at least one population of dopaminergic CNS neurons. Its role in the PNS and CNS is further substantiated by its expression pattern in the proximity of these neurons.

None

Artemin antibody was purified by affinity chromatography employing immobilized Artemin matrix.

ELISA:
Indirect:
To detect hArtemin by indirect ELISA (using 100 μ L/well antibody solution) a concentration of 0.5 - 2.0 μ g/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hArtemin.
Sandwich:
To detect hArtemin by sandwich ELISA (using 100 μ L/well antibody solution) a concentration of 0.5 - 2.0 μ g/mL of this antibody is required.
Western Blot:
To detect hArtemin by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μ g/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hArtemin is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Polyclonal

Homo sapiens

Centrifuge vial prior to opening.