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Galectin-3 Antibody

ArtNr PRS-38-170-0.1mg
Hersteller ProSci
Menge 0.1 mg
Kategorie
Typ Antibody Primary
Format Lyophilized
Applikationen WB, IHC, ELISA
Specific against Human (Homo sapiens)
Host Rabbit
Konjugat/Tag Unconjugated
NCBI LGALS3
ECLASS 10.1 32160702
ECLASS 11.0 32160702
UNSPSC 12352203
Alias L31,GAL3,MAC2,CBP35,GALBP,GALIG,LGALS2,Galectin-3,35 kDa lectin,Gal-3
Versandbedingung Raumtemperatur
Lieferbar
Manufacturer - Type
Primary Antibodies, Antibody Pairs
Shipping Temperature
Room Temp
Storage Conditions
Galectin-3 antibody is stable for at least 2 years from date of receipt at -20˚ C. The reconstituted antibody is stable for at least two weeks at 2-8˚ C. Frozen aliquots are stable for at least 6 months when stored at -20˚ C. Avoid repeated freeze-thaw cycles.
Manufacturer - Research Area
Neuroscience, , Antibody Pairs
Background
Galectins are a new family of animal lectins which appear to exhibit a variety of biological functions. Lectins, of either plant or animal origin, are carbohydrate binding proteins that interact with glycoprotein and glycolipids on the surface of animal cells.
By Type
Neuroscience
Modifications
None
Protein Gi #
215274262
Concentration
batch dependent
Application Note
Sandwich ELISA:
To detect Galectin-3 by sandwich ELISA (using 100 μ L/well antibody solution) a concentration of 0.5 - 2.0 μ g/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our biotinylated Anti-Human Galectin-3 as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Galectin-3.
Western Blot:
To detect Galectin-3 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μ g/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant Galectin-3 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Immunohistochemistry:
This antibody stained formalin-fixed paraffin-embedded sections of human pancreas infiltrating ductal adenocarcinoma tissue. The recommended concentration is 31.25 ng/mL with an overnight incubation at 4˚ C. An HRP-labeled polymer detection system was used with a DAB chromogen. Optimal results for these conditions were achieved with heat induced antigen retrieval with a pH 6.0 sodium citrate buffer. Optimal concentrations and conditions may vary.
Immunogen
Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant Galectin-3. Human Galectin-3 specific antibody was purified by affinity chromatography employing immobilized Galectin-3 matrix.
Clonality
Polyclonal
NCBI Official Name
lectin, galactoside-binding, soluble, 3
NCBI Organism
Homo sapiens
Disclaimer
This product is for research use only.
User Note
Centrifuge vial prior to opening.

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

Menge: 0.1 mg
Lieferbar: In stock
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