VEGF Antibody

ELISA, WB, Neutralization Assay

VEGF antibody is stable for at least 2 years from date of receipt at -20˚ C. The reconstituted antibody is stable for at least two weeks at 2-8˚ C. Frozen aliquots are stable for at least 6 months when stored at -20˚ C. Avoid repeated freeze-thaw cycles.

Signal Transduction, Growth Factors

17380502

Neutralization:
To yield one-half maximal inhibition [ND50] of the biological activity of Rat VEGF (10 ng/mL), a concentration of < 0.1 μ g/mL of this antibody is required.ELISA:
Indirect:
To detect Rat VEGF by indirect ELISA (using 100 μ L/well antibody solution) a concentration of 0.5 - 2.0 μ g/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Rat VEGF.
Sandwich
To detect Rat VEGF by sandwich ELISA (using 100 μ L/well antibody solution) a concentration of 0.5 - 2.0 μ g/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our biotinylated Anti-Rat VEGF as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Rat VEGF.
Western Blot:
To detect Rat VEGF by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μ g/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant Rat VEGF is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Polyclonal

Rattus norvegicustus norvegicus

Centrifuge vial prior to opening.