Leptin Antibody [G1_4B6.3]

ELISA, WB, Neutralization Assay

Leptin lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20˚ C. Reconstitute in sterile water to a concentration of 0.1 - 1.0 mg/mL. The antibody is stable for at least six weeks at 2-4˚ C when reconstituted.

Leptin plays a critical role in the regulation of body weight by inhibiting food intake and stimulating energy expenditure. Defects in Leptin production cause severe hereditary obesity in rodents and humans. In addition to its effects on body weight, leptin has a variety of other functions, including the regulation of hematopoiesis, angiogenesis, wound healing, and the immune and inflammatory response. The Leptin gene is the human homolog of the gene (ob) mutant in the mouse 'obese' phenotype.

Was product number XP-5712-M

None

This IgG1K antibody was purified from cell culture by Protein A affinity chromatography.

Neutralization:
To yield one-half maximal inhibition [ND50] of the biological activity of mIL-2 (1.0 ng/mL), a concentration of 10.0-14.0 μ g/mL of this antibody is required. ELISA:
In a sandwich ELISA (assuming 100 μ L/well), a concentration of 4.0-8.0 μ g/mL of this antibody will detect at least 32 pg/mL of recombinant murine IL-2 when used with our biotinylated antigen affinity purified anti-murine IL-2 (XP-5183Bt) as the detection antibody at a concentration of at least 1.0-2.0 μ g/mL.
Western Blot:
To detect mIL-2 by Western Blot analysis this antibody can be used at a concentration of 2.0-4.0 μ g/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant mIL-2 is 1.0-2.0 ng/lane, under reducing conditions and 0.2-0.4 ng/lane, under non-reducing conditions.

Monoclonal

Homo sapiens

Centrifuge vial prior to opening.