bNGF Antibody [M85-2B]

Primary Antibodies, Functional Antibodies

Room Temp

Neuroscience, Functional Assays

None

This IgG2bK antibody was purified from cell culture by Protein G affinity chromatography.

Neutralization:
To yield one-half maximal inhibition [ND50] of the biological activity of hB-NGF (10.0 ng/mL), a concentration of 2.7 - 4.0 μ g/mL of this antibody is required.
ELISA:
In a sandwich ELISA (assuming 100 μ L/well), a concentration of 2.0-4.0 μ g/mL of this antibody will detect at least 200 pg/mL of recombinant human β -NGF when used with our biotinylated antigen affinity purified anti-human b-NGF as the detection antibody at a concentration of approximately 0.5-1.0 μ g/mL.
Western Blot:
To detect hβ -NGF by Western Blot analysis this antibody can be used at a concentration of 4.0-8.0 μ g/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hβ -NGF is 4.0-8.0 ng/lane, under reducing or non-reducing conditions.

Monoclonal

Homo sapiens

Centrifuge vial prior to opening.